Editor’s Note: Previous studies have shown that palm oil tocotrienols, less common but ultra-potent forms of vitamin E abundant in Malaysian sustainable palm oil, may help brains recover from stroke damage. This Japanese study affirms those findings and helps explain how this nutrient might deliver its neuroprotective benefits. EVNol SupraBio, the supplement used in the study, is sourced from responsibly grown and produced Malaysian palm oil.
EVNol SupraBio shows neuroprotective effects in post-ischemic mouse brain
Pretreatment of EVNol SupraBio shows neuroprotective effects in the cerebral ischemia/reperfusion injury with direct effects on oxidative stress and advanced glycation end products (AGEs) reductions, and indirect effects on Nrf2 and MRP1 activations.
In this interesting study, Prof Koji Abe and his team of researchers at the Department of Neurology, Okayama University – Japan, supplemented a group of mice with EVNol SupraBio (encapsulated in a soft gel capsule, branded as Tocovid) once a day. After one month of supplementation, the mice were induced for ischemic stroke via transient middle cerebral artery occlusion (tMCAO) for 60 minutes. The oxidative stress, apoptosis, and autophagy cell death in the peri-ischemic lesion on day 1, 3 and 7 after tMCAO were then evaluated. Two other groups of mice were used as control, one as a vehicle control (treatment/supplement control) and one as a sham control (surgery control).
Mobile activity based on rotarod time worsened after tMCAO in the vehicle group. However, pre-supplementation with EVNol SupraBio resulted in a significant recovery of mobile activity on day one after tMCAO. Large infarcts of the lateral cortex and the underlying caudoputamen (equivalent to “dorsal striatum” in humans, a vital part of the circuitry for decision making and reward-related behavior) were observed upon tMCAO in all groups. What was compelling is that the infarcts volume was significantly reduced on day one and day three after tMCAO in the mice group receiving EVNol SupraBio.
The number of peroxidative damages to lipids (4-HNE), proteins (nitrotyrosine) and DNA (8-OHdG) were also significantly decreased in the EVNol SupraBio pretreatment group. The reduced expression of AGEs biomarkers, RAGE, CMA (vascular) and CML (neuronal) in EVNol SupraBio group confirmed the neuroprotective effect of EVNol SupraBio in ischemic mice. In addition, EVNol SupraBio pretreatment individually up-regulated Nrf2 protein level (a protein important for regulating antioxidant defense) on day seven after tMCAO.
Under oxidative stress, GSH is rapidly oxidized to GSSG which is toxic to the cells. It was observed that GSSG level significantly increased after tMCAO, resulting in higher GSSG/GSH ratio. In this study, the group of mice receiving EVNol SupraBio showed an increase in MRP1 level and reduced GSSG/GSH ratio, suggesting that EVNol SupraBio may protect against the damages caused by ischemic stroke through induction of MRP1. Supplementation with EVNol SupraBio also significantly reduced cleaved caspase-3 and LC3-II expressions, which both play an important role in autophagy and apoptosis of the cell.
The researcher concluded that EVNol SupraBio pretreatment obviously has neuroprotective activities against oxidative stress, and at least in part by antiapoptotic/autophagic cell death in ischemic mice brain.
“The neuroprotective activities of EVNol SupraBio on pre-stroke and post-stroke settings have been reported in various in vitro and clinical studies, conducted at the Ohio State University (OSU), Wexner Medical Center (funded by the U.S. National Institutes of Health) and University Science Malaysia (USM). In fact, the study of EVNol SupraBio on brain protection started for almost 20 years, all with promising and ground-breaking results. Some of the findings from this particular study aligned with and confirmed the earlier works conducted at OSU and USM and thus further strengthen the significant effectiveness of EVNol SupraBio in neuroprotection. I am extremely excited as it underscores EVNol SupraBio as a potential well-rounded and natural therapeutic agent for brain protection,” said ExcelVite Nutritionist Diyanah Roslan.
Disclaimer: The statements in the above article have not been evaluated by the Food and Drug Administration. They are not intended to diagnose, treat, cure or prevent any disease.
Source: Xcelvite press release